• • Based on widely used BHK-21
  • • Existing plasmids and transfection processes for rAAV production
  • • BHK origin avoids ethical concerns
Download our fact sheet

Cell lines for research, biomanufacturing and viral vector production

Agathos BHK-[wt E1] has been developed for recombinant adeno-associated virus (rAAV) production and other biomanufacturing and research applications that benefit from a mammalian cell expressing the adenovirus E1 gene.

BHK-[wt E1] was developed by transfecting adenovirus E1 genes with selective pressure using hygromycin.

Using existing plasmids and triple transfection techniques BHK-[wt E1] was used to produce rAAV.

rAAV2-GFP genomes from triple transfection of BHK-[wt E1] cells are detected via digital PCR (QIAGEN Qiacuity)

Fluorescent (top) and fluorescent merged with bright field (bottom) images of HepG2 cells transduced with rAAV2-GFP from BHK-[wt E1].

Send us a message

"*" indicates required fields

* Mandatory field
This field is for validation purposes and should be left unchanged.

By submitting this form you agree to Agathos holding the information securely and for us to send you information we think may be of interest. We will not share or sell your information with other companies or organizations.