rAAV2-GFP genomes from triple transfection of AE1-BHK cells are detected via digital PCR (QIAGEN Qiacuity)
AE1-BHK cell line
- • Based on widely used BHK-21
- • Existing plasmids and transfection processes for rAAV production
- • BHK origin avoids ethical concerns
Cell lines for research, biomanufacturing and viral vector production
Agathos AE1-BHK has been developed for recombinant adeno-associated virus (rAAV) production and other biomanufacturing and research applications that benefit from a mammalian cell expressing the adenovirus E1 gene.
AE1-BHK was developed by transfecting adenovirus E1 genes with selective pressure using hygromycin.
Using existing plasmids and triple transfection techniques AE1-BHK was used to produce rAAV.
Fluorescent (top) and fluorescent merged with bright field (bottom) images of HepG2 cells transduced with rAAV2-GFP from AE1-BHK.
Beyond HEK293
Ethics Meets Excellence
Choosing AE1-BHK for Your Research Needs:
Agathos Biologics is at the forefront of ethical biomanufacturing. We developed the AE1-BHK cell line to avoid ethical concerns that arise from using cells derived from embryonic stem cells or aborted fetal tissue as is the case for HEK293. Consumers and researchers alike are becoming more aware of how products and services are delivered and are demanding companies provide not just quality, but also principled business practices. Embrace a conscientious choice for your research needs, where ethics seamlessly align with exceptional products, setting a new industry standard for research and biomanufacturing.
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